scvi.model.CondSCVI#

class scvi.model.CondSCVI(adata, n_hidden=128, n_latent=5, n_layers=2, weight_obs=False, dropout_rate=0.05, **module_kwargs)[source]#

Conditional version of single-cell Variational Inference, used for multi-resolution deconvolution of spatial transcriptomics data [Lopez et al., 2021].

Parameters:
  • adata (AnnData) – AnnData object that has been registered via setup_anndata().

  • n_hidden (int) – Number of nodes per hidden layer.

  • n_latent (int) – Dimensionality of the latent space.

  • n_layers (int) – Number of hidden layers used for encoder and decoder NNs.

  • weight_obs (bool) – Whether to reweight observations by their inverse proportion (useful for lowly abundant cell types)

  • dropout_rate (float) – Dropout rate for neural networks.

  • **module_kwargs – Keyword args for VAEC

Examples

>>> adata = anndata.read_h5ad(path_to_anndata)
>>> scvi.model.CondSCVI.setup_anndata(adata, "labels")
>>> vae = scvi.model.CondSCVI(adata)
>>> vae.train()
>>> adata.obsm["X_CondSCVI"] = vae.get_latent_representation()

Attributes table#

adata

Data attached to model instance.

adata_manager

Manager instance associated with self.adata.

device

The current device that the module's params are on.

history

Returns computed metrics during training.

is_trained

Whether the model has been trained.

test_indices

Observations that are in test set.

train_indices

Observations that are in train set.

validation_indices

Observations that are in validation set.

Methods table#

convert_legacy_save(dir_path, output_dir_path)

Converts a legacy saved model (<v0.15.0) to the updated save format.

differential_expression([adata, groupby, ...])

.

get_anndata_manager(adata[, required])

Retrieves the AnnDataManager for a given AnnData object specific to this model instance.

get_elbo([adata, indices, batch_size])

Return the ELBO for the data.

get_feature_correlation_matrix([adata, ...])

Generate gene-gene correlation matrix using scvi uncertainty and expression.

get_from_registry(adata, registry_key)

Returns the object in AnnData associated with the key in the data registry.

get_latent_library_size([adata, indices, ...])

Returns the latent library size for each cell.

get_latent_representation([adata, indices, ...])

Return the latent representation for each cell.

get_likelihood_parameters([adata, indices, ...])

Estimates for the parameters of the likelihood \(p(x \mid z)\).

get_marginal_ll([adata, indices, ...])

Return the marginal LL for the data.

get_normalized_expression([adata, indices, ...])

Returns the normalized (decoded) gene expression.

get_reconstruction_error([adata, indices, ...])

Return the reconstruction error for the data.

get_vamp_prior([adata, p])

Return an empirical prior over the cell-type specific latent space (vamp prior) that may be used for deconvolution.

load(dir_path[, adata, use_gpu, prefix, ...])

Instantiate a model from the saved output.

load_registry(dir_path[, prefix])

Return the full registry saved with the model.

posterior_predictive_sample([adata, ...])

Generate observation samples from the posterior predictive distribution.

register_manager(adata_manager)

Registers an AnnDataManager instance with this model class.

save(dir_path[, prefix, overwrite, save_anndata])

Save the state of the model.

setup_anndata(adata[, labels_key, layer])

Sets up the AnnData object for this model.

to_device(device)

Move model to device.

train([max_epochs, lr, use_gpu, train_size, ...])

Trains the model using MAP inference.

view_anndata_setup([adata, ...])

Print summary of the setup for the initial AnnData or a given AnnData object.

view_setup_args(dir_path[, prefix])

Print args used to setup a saved model.

Attributes#

adata

CondSCVI.adata[source]#

Data attached to model instance.

adata_manager

CondSCVI.adata_manager[source]#

Manager instance associated with self.adata.

device

CondSCVI.device[source]#

The current device that the module’s params are on.

history

CondSCVI.history[source]#

Returns computed metrics during training.

is_trained

CondSCVI.is_trained[source]#

Whether the model has been trained.

test_indices

CondSCVI.test_indices[source]#

Observations that are in test set.

train_indices

CondSCVI.train_indices[source]#

Observations that are in train set.

validation_indices

CondSCVI.validation_indices[source]#

Observations that are in validation set.

Methods#

convert_legacy_save

classmethod CondSCVI.convert_legacy_save(dir_path, output_dir_path, overwrite=False, prefix=None)[source]#

Converts a legacy saved model (<v0.15.0) to the updated save format.

Parameters:
  • dir_path (str) – Path to directory where legacy model is saved.

  • output_dir_path (str) – Path to save converted save files.

  • overwrite (bool) – Overwrite existing data or not. If False and directory already exists at output_dir_path, error will be raised.

  • prefix (Optional[str]) – Prefix of saved file names.

Return type:

None

differential_expression

CondSCVI.differential_expression(adata=None, groupby=None, group1=None, group2=None, idx1=None, idx2=None, mode='change', delta=0.25, batch_size=None, all_stats=True, batch_correction=False, batchid1=None, batchid2=None, fdr_target=0.05, silent=False, **kwargs)[source]#

.

A unified method for differential expression analysis.

Implements 'vanilla' DE [Lopez et al., 2018] and 'change' mode DE [Boyeau et al., 2019].

adata

AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

groupby

The key of the observations grouping to consider.

group1

Subset of groups, e.g. ['g1', 'g2', 'g3'], to which comparison shall be restricted, or all groups in groupby (default).

group2

If None, compare each group in group1 to the union of the rest of the groups in groupby. If a group identifier, compare with respect to this group.

idx1

idx1 and idx2 can be used as an alternative to the AnnData keys. Custom identifier for group1 that can be of three sorts: (1) a boolean mask, (2) indices, or (3) a string. If it is a string, then it will query indices that verifies conditions on adata.obs, as described in pandas.DataFrame.query() If idx1 is not None, this option overrides group1 and group2.

idx2

Custom identifier for group2 that has the same properties as idx1. By default, includes all cells not specified in idx1.

mode

Method for differential expression. See user guide for full explanation.

delta

specific case of region inducing differential expression. In this case, we suppose that \(R \setminus [-\delta, \delta]\) does not induce differential expression (change model default case).

batch_size

Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

all_stats

Concatenate count statistics (e.g., mean expression group 1) to DE results.

batch_correction

Whether to correct for batch effects in DE inference.

batchid1

Subset of categories from batch_key registered in setup_anndata, e.g. ['batch1', 'batch2', 'batch3'], for group1. Only used if batch_correction is True, and by default all categories are used.

batchid2

Same as batchid1 for group2. batchid2 must either have null intersection with batchid1, or be exactly equal to batchid1. When the two sets are exactly equal, cells are compared by decoding on the same batch. When sets have null intersection, cells from group1 and group2 are decoded on each group in group1 and group2, respectively.

fdr_target

Tag features as DE based on posterior expected false discovery rate.

silent

If True, disables the progress bar. Default: False.

**kwargs

Keyword args for scvi.model.base.DifferentialComputation.get_bayes_factors()

Differential expression DataFrame.

Parameters:
Return type:

DataFrame

get_anndata_manager

CondSCVI.get_anndata_manager(adata, required=False)[source]#

Retrieves the AnnDataManager for a given AnnData object specific to this model instance.

Requires self.id has been set. Checks for an AnnDataManager specific to this model instance.

Parameters:
  • adata (Union[AnnData, MuData]) – AnnData object to find manager instance for.

  • required (bool) – If True, errors on missing manager. Otherwise, returns None when manager is missing.

Return type:

Optional[AnnDataManager]

get_elbo

CondSCVI.get_elbo(adata=None, indices=None, batch_size=None)[source]#

Return the ELBO for the data.

The ELBO is a lower bound on the log likelihood of the data used for optimization of VAEs. Note, this is not the negative ELBO, higher is better.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Return type:

float

get_feature_correlation_matrix

CondSCVI.get_feature_correlation_matrix(adata=None, indices=None, n_samples=10, batch_size=64, rna_size_factor=1000, transform_batch=None, correlation_type='spearman')[source]#

Generate gene-gene correlation matrix using scvi uncertainty and expression.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • n_samples (int) – Number of posterior samples to use for estimation.

  • batch_size (int) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

  • rna_size_factor (int) – size factor for RNA prior to sampling gamma distribution.

  • transform_batch (Optional[Sequence[Union[int, float, str]]]) –

    Batches to condition on. If transform_batch is:

    • None, then real observed batch is used.

    • int, then batch transform_batch is used.

    • list of int, then values are averaged over provided batches.

  • correlation_type (Literal['spearman', 'pearson']) – One of “pearson”, “spearman”.

Returns:

Gene-gene correlation matrix

Return type:

DataFrame

get_from_registry

CondSCVI.get_from_registry(adata, registry_key)[source]#

Returns the object in AnnData associated with the key in the data registry.

AnnData object should be registered with the model prior to calling this function via the self._validate_anndata method.

Parameters:
  • registry_key (str) – key of object to get from data registry.

  • adata (Union[AnnData, MuData]) – AnnData to pull data from.

Returns:

The requested data as a NumPy array.

Return type:

ndarray

get_latent_library_size

CondSCVI.get_latent_library_size(adata=None, indices=None, give_mean=True, batch_size=None)[source]#

Returns the latent library size for each cell.

This is denoted as \(\ell_n\) in the scVI paper.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • give_mean (bool) – Return the mean or a sample from the posterior distribution.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Return type:

ndarray

get_latent_representation

CondSCVI.get_latent_representation(adata=None, indices=None, give_mean=True, mc_samples=5000, batch_size=None, return_dist=False)[source]#

Return the latent representation for each cell.

This is typically denoted as \(z_n\).

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • give_mean (bool) – Give mean of distribution or sample from it.

  • mc_samples (int) – For distributions with no closed-form mean (e.g., logistic normal), how many Monte Carlo samples to take for computing mean.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

  • return_dist (bool) – Return (mean, variance) of distributions instead of just the mean. If True, ignores give_mean and mc_samples. In the case of the latter, mc_samples is used to compute the mean of a transformed distribution. If return_dist is true the untransformed mean and variance are returned.

Returns:

Low-dimensional representation for each cell or a tuple containing its mean and variance.

Return type:

Union[ndarray, Tuple[ndarray, ndarray]]

get_likelihood_parameters

CondSCVI.get_likelihood_parameters(adata=None, indices=None, n_samples=1, give_mean=False, batch_size=None)[source]#

Estimates for the parameters of the likelihood \(p(x \mid z)\).

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • n_samples (Optional[int]) – Number of posterior samples to use for estimation.

  • give_mean (Optional[bool]) – Return expected value of parameters or a samples

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Return type:

Dict[str, ndarray]

get_marginal_ll

CondSCVI.get_marginal_ll(adata=None, indices=None, n_mc_samples=1000, batch_size=None)[source]#

Return the marginal LL for the data.

The computation here is a biased estimator of the marginal log likelihood of the data. Note, this is not the negative log likelihood, higher is better.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • n_mc_samples (int) – Number of Monte Carlo samples to use for marginal LL estimation.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Return type:

float

get_normalized_expression

CondSCVI.get_normalized_expression(adata=None, indices=None, transform_batch=None, gene_list=None, library_size=1, n_samples=1, n_samples_overall=None, batch_size=None, return_mean=True, return_numpy=None)[source]#

Returns the normalized (decoded) gene expression.

This is denoted as \(\rho_n\) in the scVI paper.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • transform_batch (Optional[Sequence[Union[int, float, str]]]) –

    Batch to condition on. If transform_batch is:

    • None, then real observed batch is used.

    • int, then batch transform_batch is used.

  • gene_list (Optional[Sequence[str]]) – Return frequencies of expression for a subset of genes. This can save memory when working with large datasets and few genes are of interest.

  • library_size (Union[float, Literal['latent']]) – Scale the expression frequencies to a common library size. This allows gene expression levels to be interpreted on a common scale of relevant magnitude. If set to "latent", use the latent library size.

  • n_samples (int) – Number of posterior samples to use for estimation.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

  • return_mean (bool) – Whether to return the mean of the samples.

  • return_numpy (Optional[bool]) – Return a ndarray instead of a DataFrame. DataFrame includes gene names as columns. If either n_samples=1 or return_mean=True, defaults to False. Otherwise, it defaults to True.

  • n_samples_overall (int) –

Returns:

If n_samples > 1 and return_mean is False, then the shape is (samples, cells, genes). Otherwise, shape is (cells, genes). In this case, return type is DataFrame unless return_numpy is True.

Return type:

Union[ndarray, DataFrame]

get_reconstruction_error

CondSCVI.get_reconstruction_error(adata=None, indices=None, batch_size=None)[source]#

Return the reconstruction error for the data.

This is typically written as \(p(x \mid z)\), the likelihood term given one posterior sample. Note, this is not the negative likelihood, higher is better.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Return type:

float

get_vamp_prior

CondSCVI.get_vamp_prior(adata=None, p=10)[source]#

Return an empirical prior over the cell-type specific latent space (vamp prior) that may be used for deconvolution.

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • p (int) – number of clusters in kmeans clustering for cell-type sub-clustering for empirical prior

Returns:

mean_vprior: np.ndarray

(n_labels, p, D) array

var_vprior

(n_labels, p, D) array

Return type:

ndarray

load

classmethod CondSCVI.load(dir_path, adata=None, use_gpu=None, prefix=None, backup_url=None)[source]#

Instantiate a model from the saved output.

Parameters:
  • dir_path (str) – Path to saved outputs.

  • adata (Optional[Union[AnnData, MuData]]) – AnnData organized in the same way as data used to train model. It is not necessary to run setup_anndata, as AnnData is validated against the saved scvi setup dictionary. If None, will check for and load anndata saved with the model.

  • use_gpu (Optional[Union[str, int, bool]]) – Load model on default GPU if available (if None or True), or index of GPU to use (if int), or name of GPU (if str), or use CPU (if False).

  • prefix (Optional[str]) – Prefix of saved file names.

  • backup_url (Optional[str]) – URL to retrieve saved outputs from if not present on disk.

Returns:

Model with loaded state dictionaries.

Examples

>>> model = ModelClass.load(save_path, adata) # use the name of the model class used to save
>>> model.get_....

load_registry

static CondSCVI.load_registry(dir_path, prefix=None)[source]#

Return the full registry saved with the model.

Parameters:
  • dir_path (str) – Path to saved outputs.

  • prefix (Optional[str]) – Prefix of saved file names.

Returns:

The full registry saved with the model

Return type:

dict

posterior_predictive_sample

CondSCVI.posterior_predictive_sample(adata=None, indices=None, n_samples=1, gene_list=None, batch_size=None)[source]#

Generate observation samples from the posterior predictive distribution.

The posterior predictive distribution is written as \(p(\hat{x} \mid x)\).

Parameters:
  • adata (Optional[AnnData]) – AnnData object with equivalent structure to initial AnnData. If None, defaults to the AnnData object used to initialize the model.

  • indices (Optional[Sequence[int]]) – Indices of cells in adata to use. If None, all cells are used.

  • n_samples (int) – Number of samples for each cell.

  • gene_list (Optional[Sequence[str]]) – Names of genes of interest.

  • batch_size (Optional[int]) – Minibatch size for data loading into model. Defaults to scvi.settings.batch_size.

Returns:

x_new : torch.Tensor tensor with shape (n_cells, n_genes, n_samples)

Return type:

ndarray

register_manager

classmethod CondSCVI.register_manager(adata_manager)[source]#

Registers an AnnDataManager instance with this model class.

Stores the AnnDataManager reference in a class-specific manager store. Intended for use in the setup_anndata() class method followed up by retrieval of the AnnDataManager via the _get_most_recent_anndata_manager() method in the model init method.

Notes

Subsequent calls to this method with an AnnDataManager instance referring to the same underlying AnnData object will overwrite the reference to previous AnnDataManager.

Parameters:

adata_manager (AnnDataManager) –

save

CondSCVI.save(dir_path, prefix=None, overwrite=False, save_anndata=False, **anndata_write_kwargs)[source]#

Save the state of the model.

Neither the trainer optimizer state nor the trainer history are saved. Model files are not expected to be reproducibly saved and loaded across versions until we reach version 1.0.

Parameters:
  • dir_path (str) – Path to a directory.

  • prefix (Optional[str]) – Prefix to prepend to saved file names.

  • overwrite (bool) – Overwrite existing data or not. If False and directory already exists at dir_path, error will be raised.

  • save_anndata (bool) – If True, also saves the anndata

  • anndata_write_kwargs – Kwargs for write()

setup_anndata

classmethod CondSCVI.setup_anndata(adata, labels_key=None, layer=None, **kwargs)[source]#

Sets up the AnnData object for this model.

A mapping will be created between data fields used by this model to their respective locations in adata. None of the data in adata are modified. Only adds fields to adata.

Parameters:
  • adata (AnnData) – AnnData object. Rows represent cells, columns represent features.

  • labels_key (Optional[str]) – key in adata.obs for label information. Categories will automatically be converted into integer categories and saved to adata.obs['_scvi_labels']. If None, assigns the same label to all the data.

  • layer (Optional[str]) – if not None, uses this as the key in adata.layers for raw count data.

to_device

CondSCVI.to_device(device)[source]#

Move model to device.

Parameters:

device (Union[str, int]) – Device to move model to. Options: ‘cpu’ for CPU, integer GPU index (eg. 0), or ‘cuda:X’ where X is the GPU index (eg. ‘cuda:0’). See torch.device for more info.

Examples

>>> adata = scvi.data.synthetic_iid()
>>> model = scvi.model.SCVI(adata)
>>> model.to_device('cpu')      # moves model to CPU
>>> model.to_device('cuda:0')   # moves model to GPU 0
>>> model.to_device(0)          # also moves model to GPU 0

train

CondSCVI.train(max_epochs=300, lr=0.001, use_gpu=None, train_size=1, validation_size=None, batch_size=128, plan_kwargs=None, **kwargs)[source]#

Trains the model using MAP inference.

Parameters:
  • max_epochs (int) – Number of epochs to train for

  • lr (float) – Learning rate for optimization.

  • use_gpu (Optional[Union[str, int, bool]]) – Use default GPU if available (if None or True), or index of GPU to use (if int), or name of GPU (if str, e.g., 'cuda:0'), or use CPU (if False).

  • train_size (float) – Size of training set in the range [0.0, 1.0].

  • validation_size (Optional[float]) – Size of the test set. If None, defaults to 1 - train_size. If train_size + validation_size < 1, the remaining cells belong to a test set.

  • batch_size (int) – Minibatch size to use during training.

  • plan_kwargs (Optional[dict]) – Keyword args for TrainingPlan. Keyword arguments passed to train() will overwrite values present in plan_kwargs, when appropriate.

  • **kwargs – Other keyword args for Trainer.

view_anndata_setup

CondSCVI.view_anndata_setup(adata=None, hide_state_registries=False)[source]#

Print summary of the setup for the initial AnnData or a given AnnData object.

Parameters:
Return type:

None

view_setup_args

static CondSCVI.view_setup_args(dir_path, prefix=None)[source]#

Print args used to setup a saved model.

Parameters:
  • dir_path (str) – Path to saved outputs.

  • prefix (Optional[str]) – Prefix of saved file names.

Return type:

None