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Multi-resolution deconvolution of spatial transcriptomics in R

In this brief tutorial, we go over how to use scvi-tools functionality in R for analyzing spatial datasets. We will load spatial data following this Seurat tutorial, subsequently analyzing the data using DestVI.

This tutorial requires Reticulate. Please check out our installation guide for instructions on installing Reticulate and scvi-tools.

Loading and processing data with Seurat

# install.packages("Seurat")
# install.packages("reticulate")
# install.packages("anndata")
# install.packages("devtools")
# devtools::install_github("satijalab/seurat-data")

# if (!requireNamespace("BiocManager", quietly = TRUE))
#     install.packages("BiocManager")

# BiocManager::install(c("LoomExperiment", "SingleCellExperiment"))
# devtools::install_github("cellgeni/sceasy")

library(Seurat)
library(SeuratData)
library(ggplot2)

Attaching SeuratObject

Registered S3 method overwritten by 'cli':
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── Installed datasets ────────────────────────────────────────────────────────────────────────────────────────── SeuratData v0.2.1 ──

✔ ifnb     3.0.0                                                  ✔ stxBrain 0.1.1
✔ pbmc3k   3.1.4


──────────────────────────────────────────────────────────────── Key ────────────────────────────────────────────────────────────────

✔ Dataset loaded successfully
❯ Dataset built with a newer version of Seurat than installed
❓ Unknown version of Seurat installed

First, we load the reference SMART-seq2 dataset of mouse brain. This dataset contains about 14,000 cells.

cortex_sc_data <- readRDS(url("https://www.dropbox.com/s/cuowvm4vrf65pvq/allen_cortex.rds?dl=1"))

InstallData("stxBrain")

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brain_st_data <- LoadData("stxBrain", type = "anterior1")

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Now, we subset the data in the same way that was done in the Seurat vignette, to match the cortex single-cell reference we are using.

brain_st_data <- SCTransform(brain_st_data, assay = "Spatial", verbose = FALSE)
brain_st_data <- RunPCA(brain_st_data, assay = "SCT", verbose = FALSE)
brain_st_data <- FindNeighbors(brain_st_data, reduction = "pca", dims = 1:30)
brain_st_data <- FindClusters(brain_st_data, verbose = FALSE)
brain_st_data <- RunUMAP(brain_st_data, reduction = "pca", dims = 1:30)

cortex_st_data <- subset(brain_st_data, idents = c(1, 2, 3, 4, 6, 7))
cortex_st_data <- subset(cortex_st_data, anterior1_imagerow > 400 | anterior1_imagecol < 150, invert = TRUE)
cortex_st_data <- subset(cortex_st_data, anterior1_imagerow > 275 & anterior1_imagecol > 370, invert = TRUE)
cortex_st_data <- subset(cortex_st_data, anterior1_imagerow > 250 & anterior1_imagecol > 440, invert = TRUE)

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Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
Warning message in theta.ml(y = y, mu = fit$fitted):
“iteration limit reached”
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Computing nearest neighbor graph

Computing SNN

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To use Python UMAP via reticulate, set umap.method to 'umap-learn' and metric to 'correlation'
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12:02:55 UMAP embedding parameters a = 0.9922 b = 1.112

12:02:55 Read 2696 rows and found 30 numeric columns

12:02:55 Using Annoy for neighbor search, n_neighbors = 30

12:02:55 Building Annoy index with metric = cosine, n_trees = 50

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12:02:56 Writing NN index file to temp file /tmp/Rtmp7FM9c5/file19ee5dfab826

12:02:56 Searching Annoy index using 1 thread, search_k = 3000

12:02:57 Annoy recall = 100%

12:02:57 Commencing smooth kNN distance calibration using 1 thread

12:02:57 Initializing from normalized Laplacian + noise

12:02:58 Commencing optimization for 500 epochs, with 105696 positive edges

12:03:07 Optimization finished

cortex_sc_data

An object of class Seurat
34617 features across 14249 samples within 1 assay
Active assay: RNA (34617 features, 0 variable features)

cortex_st_data

An object of class Seurat
48721 features across 1073 samples within 2 assays
Active assay: SCT (17668 features, 3000 variable features)
 1 other assay present: Spatial
 2 dimensional reductions calculated: pca, umap

cortex_sc_data <- NormalizeData(cortex_sc_data, normalization.method = "LogNormalize", scale.factor = 10000)
cortex_sc_data <- FindVariableFeatures(cortex_sc_data, selection.method = "vst", nfeatures = 2000)
top2000 <- head(VariableFeatures(cortex_sc_data), 2000)
top2000intersect <- intersect(rownames(cortex_st_data), top2000)

cortex_sc_data <- cortex_sc_data[top2000intersect]
cortex_st_data <- cortex_st_data[top2000intersect]
G <- length(top2000intersect)
G

1658

SpatialFeaturePlot(cortex_st_data, features = "nCount_Spatial") + theme(legend.position = "right")

Warning message:
“`guides(<scale> = FALSE)` is deprecated. Please use `guides(<scale> = "none")` instead.”

Data conversion (Seurat -> AnnData)

We use sceasy for conversion, and load the necessary Python packages for later.

library(reticulate)
library(sceasy)
library(anndata)

sc <- import("scanpy", convert = FALSE)
scvi <- import("scvi", convert = FALSE)

We make two AnnData objects, one for the single-cell reference and one for the spatial transcriptomics data, and then move the measurement coordinates to the appropriate attribute of the spatial AnnData.

cortex_sc_adata <- convertFormat(cortex_sc_data, from="seurat", to="anndata", main_layer="counts", drop_single_values=FALSE)
cortex_st_adata <- convertFormat(cortex_st_data, from="seurat", to="anndata", assay="Spatial", main_layer="counts", drop_single_values=FALSE)

Fit the scLVM

scvi$model$CondSCVI$setup_anndata(cortex_sc_adata, labels_key="subclass")

None

Here we would like to reweight each measurement by a scalar factor (e.g., the inverse proportion) in the loss of the model so that lowly abundant cell types get better fit by the model.

sclvm <- scvi$model$CondSCVI(cortex_sc_adata, weight_obs=TRUE)
sclvm$train(max_epochs=as.integer(250))

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# Make plot smaller.
saved <- options(repr.plot.width=6, repr.plot.height=5)

sclvm_elbo <- py_to_r(sclvm$history["elbo_train"]$astype("float64"))
ggplot(data = sclvm_elbo, mapping = aes(x=as.numeric(rownames(sclvm_elbo)), y=elbo_train)) + geom_line() + xlab("Epoch") + ylab("ELBO") + xlim(10, NA)

# Revert plot settings.
options(saved)

Warning message:
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Deconvolution with stLVM

scvi$model$DestVI$setup_anndata(cortex_st_adata)

None

stlvm <- scvi$model$DestVI$from_rna_model(cortex_st_adata, sclvm)
stlvm$train(max_epochs=as.integer(2500))

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# Make plot smaller.
saved <- options(repr.plot.width=6, repr.plot.height=5)

stlvm_elbo <- py_to_r(stlvm$history["elbo_train"]$astype("float64"))
ggplot(data = stlvm_elbo, mapping = aes(x=as.numeric(rownames(stlvm_elbo)), y=elbo_train)) + geom_line() + xlab("Epoch") + ylab("ELBO") + xlim(10, NA)

# Revert plot settings.
options(saved)

Warning message:
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Cell type proportions

cortex_st_adata$obsm["proportions"] <- stlvm$get_proportions()

head(py_to_r(cortex_st_adata$obsm$get("proportions")))

A data.frame: 6 × 23

	Astro	CR	Endo	L2/3 IT	L4	L5 IT	L5 PT	L6 CT	L6 IT	L6b	⋯	NP	Oligo	Peri	Pvalb	SMC	Serpinf1	Sncg	Sst	VLMC	Vip
	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	⋯	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>	<dbl>
AAACAGAGCGACTCCT-1	0.132741362	0.008747282	0.011718361	0.221762791	0.0070496136	0.1546385437	0.077167749	0.0005695362	0.069925420	0.152741432	⋯	0.0007827459	0.010729197	0.023100458	0.0039191837	0.010946345	0.0147322891	0.0147522716	0.001043379	0.003957638	0.0138991615
AAACCGGGTAGGTACC-1	0.184176832	0.003791695	0.005254562	0.234996960	0.0800886825	0.0067960601	0.048472349	0.0326679721	0.091120958	0.001587714	⋯	0.0635546148	0.001604313	0.002388439	0.0015827275	0.010713122	0.0008687067	0.0090936236	0.063255355	0.055150922	0.0461300276
AAACCGTTCGTCCAGG-1	0.012769192	0.018516796	0.084555522	0.002121322	0.0692796335	0.0043616821	0.001594895	0.0711863041	0.003215992	0.009484144	⋯	0.0029538164	0.023589995	0.018594360	0.0465325005	0.199640453	0.0400414765	0.0009976953	0.051015414	0.267024368	0.0210210774
AAACTCGTGATATAAG-1	0.001966703	0.001308091	0.003387878	0.001047674	0.0006783445	0.0022771859	0.001468480	0.1649082154	0.005263278	0.601306736	⋯	0.0017696184	0.176176697	0.001715337	0.0002066867	0.001542291	0.0004248595	0.0003984653	0.000195155	0.001623075	0.0003795118
AAAGGGATGTAGCAAG-1	0.092700399	0.000697523	0.020046912	0.127878055	0.1897880286	0.1099940836	0.077412799	0.0014952135	0.006032379	0.001297700	⋯	0.0023012036	0.017050896	0.001693511	0.1491737664	0.018952442	0.0049221730	0.0014064765	0.112875126	0.014681718	0.0166219342
AAATAACCATACGGGA-1	0.147560343	0.008129421	0.033958640	0.391459972	0.0033571674	0.0007401044	0.016744409	0.0909470767	0.146570548	0.004010124	⋯	0.0007179513	0.004490611	0.004775111	0.0008306661	0.001726551	0.0103975814	0.0006136570	0.001548895	0.005978103	0.0521079451

cortex_st_data[["predictions"]] <- CreateAssayObject(data = t(py_to_r(cortex_st_adata$obsm$get("proportions"))))

DefaultAssay(cortex_st_data) <- "predictions"
SpatialFeaturePlot(cortex_st_data, features = c("L2/3 IT", "L4"), pt.size.factor = 1.6, ncol = 2, crop = TRUE)

Warning message:
“`guides(<scale> = FALSE)` is deprecated. Please use `guides(<scale> = "none")` instead.”
Warning message:
“`guides(<scale> = FALSE)` is deprecated. Please use `guides(<scale> = "none")` instead.”

Intra cell type information

At the heart of DestVI is a multitude of latent variables (5 per cell type per spots). We refer to them as “gamma”, and we may manually examine them for downstream analysis.

Because those values may be hard to examine for end-users, we presented several methods for prioritizing the study of different cell types (based on PCA and Hotspot). If you’d like to use those methods, please refer to our DestVI reproducibility repository. If you have suggestions to improve those, and would like to see them in the main codebase, reach out to us.

In this tutorial, we assume that the user have identified key gene modules that vary within one cell type in the single-cell RNA sequencing data (e.g., using Hotspot). We provide here a code snippet for imputing the spatial pattern of the cell type specific gene expression, using the example of the PLP1 gene in Endothelial cells.

for (cell_type_gamma in iterate(stlvm$get_gamma()$items())) {
    cell_type <- cell_type_gamma[0]
    gamma_df <- cell_type_gamma[1]
    cortex_st_data[[paste(cell_type, "_gamma", sep = "")]] <- CreateAssayObject(data = t(py_to_r(gamma_df)))
}

Warning message:
“Invalid name supplied, making object name syntactically valid. New object name is L2.3.ITgamma; see ?make.names for more details on syntax validity”
Warning message:
“Keys should be one or more alphanumeric characters followed by an underscore, setting key from l2.3.itgamma_ to l23itgamma_”
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“Invalid name supplied, making object name syntactically valid. New object name is L5.ITgamma; see ?make.names for more details on syntax validity”
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“Keys should be one or more alphanumeric characters followed by an underscore, setting key from l5.itgamma_ to l5itgamma_”
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“Invalid name supplied, making object name syntactically valid. New object name is L5.PTgamma; see ?make.names for more details on syntax validity”
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“Keys should be one or more alphanumeric characters followed by an underscore, setting key from l5.ptgamma_ to l5ptgamma_”
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Warning message:
“Keys should be one or more alphanumeric characters followed by an underscore, setting key from l6.ctgamma_ to l6ctgamma_”
Warning message:
“Invalid name supplied, making object name syntactically valid. New object name is L6.ITgamma; see ?make.names for more details on syntax validity”
Warning message:
“Keys should be one or more alphanumeric characters followed by an underscore, setting key from l6.itgamma_ to l6itgamma_”

head(GetAssayData(cortex_st_data))

A matrix: 6 × 1073 of type dbl

	AAACAGAGCGACTCCT-1	AAACCGGGTAGGTACC-1	AAACCGTTCGTCCAGG-1	AAACTCGTGATATAAG-1	AAAGGGATGTAGCAAG-1	AAATAACCATACGGGA-1	AAATCGTGTACCACAA-1	AAATGATTCGATCAGC-1	AAATGGTCAATGTGCC-1	AAATTAACGGGTAGCT-1	⋯	TTGGTCACACTCGTAA-1	TTGTAAGGACCTAAGT-1	TTGTAAGGCCAGTTGG-1	TTGTAATCCGTACTCG-1	TTGTCGTTCAGTTACC-1	TTGTGGCCCTGACAGT-1	TTGTGTTTCCCGAAAG-1	TTGTTCAGTGTGCTAC-1	TTGTTGTGTGTCAAGA-1	TTGTTTCCATACAACT-1
Astro	0.132741362	0.184176832	0.012769192	0.0019667035	0.092700399	0.1475603431	0.0004045009	0.031076301	0.136070669	0.0197930187	⋯	0.1365053356	0.08824219	0.087588087	0.081193000	0.1984594613	0.281091094	0.016215326	0.081116490	0.061543569	0.1241387874
CR	0.008747282	0.003791695	0.018516796	0.0013080909	0.000697523	0.0081294207	0.0063187955	0.003309071	0.003154238	0.0008858569	⋯	0.0037494451	0.03061977	0.003443674	0.001939139	0.0097711813	0.003028526	0.026655646	0.001710637	0.005380775	0.1895391792
Endo	0.011718361	0.005254562	0.084555522	0.0033878782	0.020046912	0.0339586399	0.0019674290	0.039658114	0.051655442	0.0185621772	⋯	0.0050291684	0.02816591	0.001338468	0.052013196	0.0486835353	0.026372865	0.012466145	0.043418083	0.046080951	0.0098529318
L2/3 IT	0.221762791	0.234996960	0.002121322	0.0010476735	0.127878055	0.3914599717	0.0094522228	0.001090960	0.052895129	0.0520999767	⋯	0.0052948804	0.16736636	0.003720999	0.004698100	0.1383295357	0.169778779	0.004306178	0.265172213	0.002287487	0.0017337656
L4	0.007049614	0.080088682	0.069279633	0.0006783445	0.189788029	0.0033571674	0.0009997272	0.097140528	0.060398243	0.0105798170	⋯	0.0224371944	0.01600794	0.146402493	0.011464869	0.1294399202	0.123409927	0.016023967	0.003908478	0.001758869	0.0001608078
L5 IT	0.154638544	0.006796060	0.004361682	0.0022771859	0.109994084	0.0007401044	0.1498650759	0.039135456	0.385494292	0.1260202378	⋯	0.0008620349	0.00132572	0.206354573	0.103019625	0.0007694541	0.002617676	0.122893602	0.002557966	0.207107291	0.0006238798

ct_name <- "L6 IT"
gene_name <- "Plp1"

# filter for spots with low abundance
indices <- which(GetAssayData(cortex_st_data$predictions)[ct_name,] > 0.03)

# impute gene
specific_expression <- stlvm$get_scale_for_ct(ct_name, indices = r_to_py(as.integer(indices - 1)))[[gene_name]]
specific_expression <- 1 + 1e4 * py_to_r(specific_expression$to_frame())

filtered_st_data <- cortex_st_data[, indices]
filtered_st_data[["imputation"]] <- CreateAssayObject(data = t(specific_expression))

DefaultAssay(filtered_st_data) <- "imputation"
SpatialFeaturePlot(filtered_st_data, features = gene_name)

Warning message:
“`guides(<scale> = FALSE)` is deprecated. Please use `guides(<scale> = "none")` instead.”

Session Info

sI <- sessionInfo()
sI$loadedOnly <- NULL
print(sI, locale=FALSE)

R version 4.0.3 (2020-10-10)
Platform: x86_64-conda-linux-gnu (64-bit)
Running under: Ubuntu 16.04.6 LTS

Matrix products: default
BLAS/LAPACK: /data/yosef2/users/jhong/miniconda3/envs/r_tutorial/lib/libopenblasp-r0.3.12.so

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
 [1] anndata_0.7.5.3           sceasy_0.0.6
 [3] reticulate_1.22           ggplot2_3.3.5
 [5] stxBrain.SeuratData_0.1.1 pbmc3k.SeuratData_3.1.4
 [7] ifnb.SeuratData_3.0.0     SeuratData_0.2.1
 [9] SeuratObject_4.0.2        Seurat_4.0.4