User

Import scvi-tools as:

import scvi

Model

model.AUTOZI	Automatic identification of ZI genes [Clivio19].
model.CondSCVI	Conditional version of single-cell Variational Inference, used for multi-resolution deconvolution of spatial transcriptomics data [Lopez21].
model.DestVI	Multi-resolution deconvolution of Spatial Transcriptomics data (DestVI) [Lopez21].
model.LinearSCVI	Linearly-decoded VAE [Svensson20].
model.PEAKVI	Peak Variational Inference [Ashuach21]
model.SCANVI	Single-cell annotation using variational inference [Xu21].
model.SCVI	single-cell Variational Inference [Lopez18].
model.TOTALVI	total Variational Inference [GayosoSteier21].
model.MULTIVI	Integration of multi-modal and single-modality data [AshuachGabitto21].

External models

external.CellAssign	Reimplementation of CellAssign for reference-based annotation [Zhang19].
external.GIMVI	Joint VAE for imputing missing genes in spatial data [Lopez19].
external.RNAStereoscope	Reimplementation of Stereoscope [Andersson20] for deconvolution of spatial transcriptomics from single-cell transcriptomics.
external.SpatialStereoscope	Reimplementation of Stereoscope [Andersson20] for deconvolution of spatial transcriptomics from single-cell transcriptomics.
external.SOLO	Doublet detection in scRNA-seq [Bernstein19].

Data loading

scvi-tools relies entirely on the AnnData format. For convenience, we have included data loaders from the AnnData API. Scanpy also has utilities to load data that are outputted by 10x’s Cell Ranger software.

data.read_h5ad	Read .h5ad-formatted hdf5 file.
data.read_csv	Read .csv file.
data.read_loom	Read .loom-formatted hdf5 file.
data.read_text	Read .txt, .tab, .data (text) file.
data.read_10x_atac	Read scATAC-seq data outputted by 10x Genomics software.
data.read_10x_multiome	Read Multiome (scRNA + scATAC) data outputted by 10x Genomics software.

Data preparation

Setting up an AnnData object is a prerequisite for running any scvi-tools model.

data.setup_anndata	Sets up AnnData object for models.
data.transfer_anndata_setup	Transfer anndata setup from a source object to a target object.
data.register_tensor_from_anndata	Add another tensor to scvi data registry.
data.view_anndata_setup	Prints setup anndata.

Basic preprocessing

For general single-cell preprocessing, we defer to our friends at Scanpy, and specifically their preprocessing module (scanpy.pp).

All scvi-tools models require raw UMI count data. The count data can be safely stored in an AnnData layer as one of the first steps of a Scanpy single-cell workflow:

adata.layers["counts"] = adata.X.copy()

Here we maintain a few package specific utilities for feature selection, etc.

data.poisson_gene_selection	Rank and select genes based on the enrichment of zero counts.
data.organize_cite_seq_10x	Organize anndata object loaded from 10x for scvi models.
data.organize_multiome_anndatas	Concatenate multiome and single-modality input anndata objects.

Configuration

An instance of the ScviConfig is available as scvi.settings and allows configuring scvi-tools.

_settings.ScviConfig

Config manager for scvi-tools.

API scvi.model.AUTOZI